Irangi ry’i Mindray 5-Igice c’Ibikoresho vyo gupima amaraso birimwo irangi ry’umuco ryitwa polyméthine n’iry’aside nucléique rifatanya canecane na ADN n’ARN y’ingingo ngenderwako. Ivyo bikoresho bishirako amazina y’utugingo ngengabuzima twera, utugingo ngengabuzima twitwa reticulocytes be n’utugingo ngengabuzima tw’amaraso turi imbere mu turemangingo tw’amaraso, ivyo bikaba bituma umuntu ashobora gutandukanya ibintu vyinshi biciye ku gushwiragira kw’umuco w’umuco w’izuba n’ugutahura ivy’umuco w’izuba.

Ivyo Gutera Amabara / Ibara ry’Amaraso Ni Ibiki?

Ivyo bikoresho bikoreshwa mu gupima amaraso ni ibisubizo vy’imiti birimwo ivyuma bita fluorophores, ivyuma bikora ku mubiri, n’ivyuma bifasha amazi gukomera. Mu buryo butandukanye n’amabara y’amaraso akoreshwa n’amaboko, ivyo bikoresho vy’ibara ry’amazi bikora imbere mu bikoresho vy’ugupima uruja n’uruza rw’amaraso.

Iyo iryo rangi ryinjijwe mu cumba c’ugutahura, rica mu nzira y’ingingo ngenderwako ziteguwe n’ibikoresho vy’ugusenyuka vy’ingingo ngenderwako maze rikafatanya mu buryo bwa stoichiometrie n’aside nucléique ziri mu ngingo ngenderwako. Iyo izo fluorophores ziboshe zishikiwe n’umuco w’umuco w’izuba uri imbere mu gice c’umubiri, zica zitanga umuco w’inyuma ugereranijwe n’ivyo aside nucléique iri mu gice c’umubiri.

Uruhara mu gushikiriza utugingo ngengabuzima / kumenya

Irangi ry'umuco rishobora gutandukanya utunyabugingo tw'amaraso:
Gushirako ikimenyetso c'aside nucléique:Ivyo bita fluorophores bica bifatanya n’imirongo ya ARN n’iya ADN iri mu turemangingo. Utugingo ngengabuzima dufise ubushobozi bwinshi bwo guhindura ibiribwa canke dufise inzira zitakura, turanywa irangi ryinshi cane, bigatuma haba ibimenyetso bikomeye vy’umuco.
Uruvyaro rwa Scattergram:Gufatanya umuco wo ku ruhande n'uburemere bw'umuco wo ku ruhande bituma haba ibishushanyo vy'ingero nyinshi-.
Gusuzuma utugingo ngengabuzima tudasanzwe:Ivyo bimenyetso bitandukanye vy’imishwarara y’umuco bitandukanya utugingo ngengabuzima tudasanzwe, utugingo ngengabuzima tutakura be n’utugingo ngengabuzima dutukura dufise utugingo ngengabuzima twera dukuze.

Imirongo yo kumenya

Umurongo wo kumenya	Izina ry'umurongo / Intumbero	Ibikorwa vy'ibinyabuzima n'uburyo bwo kubipima
Umurongo wa WNR	Utugingo ngengabuzima twera dufise umurongo wa RBC	Ipima igitigiri cose c’amaraso y’umubiri n’amaraso y’umubiri (NRBC). Basophiles zitandukanywa biciye mu gushwiragira kw’umubiri mu gihe aside nucleique ya NRBC zifise ikimenyetso c’umuco.
Umurongo wa WDF	Umurongo w'itandukaniro ry'utugingo ngengabuzima twera	Itandukanya ama Neutrophiles, Lymphocytes, Monocytes n’Eosinophiles. Ipima Granulocytes zitakura biciye ku bukomezi bw’ugufatanya kw’irangi ry’umuco w’aside nucléique.
Umurongo wa WPC	Umurongo w'imbere w'agace k'umweru	Ivyuma bipima utugingo ngengabuzima tw’umuvyeyi, utugingo ngengabuzima tudasanzwe, n’ibiturika. Ikoresha uburyo bwihariye bwo guca mu ruhu bufatanijwe n’ugufata irangi kugira ngo itandukanye utugingo ngengabuzima tw’ibiturika.
Umurongo wa RET	Umurongo w'amaraso	Ipima igipimo c’utugingo ngengabuzima, igiharuro c’amaraso, igice c’utugingo ngengabuzima tutakura, n’ibipimo bingana n’utugingo ngengabuzima dutukura biciye ku gutera ibara ry’umuco wa ARN.
Umurongo wa PLT	Umurongo w'amaso w'utugingo ngengabuzima	Itanga igiharuro c’amaplateleti gikoresheje amaso ikoresheje irangi ry’umuco ry’ARN y’amaplateleti, bikabuza guhungabanywa n’utugingo ngengabuzima dutoduto canke ibisigazwa.

Urutonde rw'ibisesangura bihuye

Urutonde rw'Ivy'Amaraso vy'Ibice 5:Ivyateguwe ku buryo bw’amazi bukoresha ubuhinga bwa laser bukoreshwa n’umuyagankuba.

Ivyiyumviro vyinshi -Ibibanza vy'akazi vy'amaraso:Ihuye n'ibikoresho vy'ugutobora ivyerekanwa vy'ivyuma - n'imirongo y'amazi iguma ishiramwo.

Ivyigwa vy'amaraso -Uburyo bwo gukora amashusho:Ivyemejwe ku buryo bwo gutanga ibikoresho bitaziguye ata guhinduka kw’ikimenyetso canke uguhinduka kw’umuvuduko.

Ibisobanuro vy'igicuruzwa

Parametere	Itegeko ry'ubuhinga / Amakuru
Ubwoko bw'imiterere	Umuti w'irangi ry'amazi ryinshi
Ishingiro rya Fluorophore rikora	Irangi ry'umuco rya polimetini / aside nucléique
Uburebure bw'umuvuduko	633 nm – 635 nm (umutuku w’umuyagankuba)
pH Urutonde (degre 25)	7.00 – 8.50
Ubushobozi bwo kuyobora umuyagankuba (degre 25)	1,20 - 2,50 mS/santimetero
Uburemere (degre 20)	1,005 - 1,020 g/cm3
Gufungura-Ugushikama kw'ikibindi	Iminsi 60 (degre 2 – degre 30 mu kubika mu mwijima)
Ubuzima butafunguwe	amezi 18
Ubushuhe bwo kubika	2 degre – 30 degre (Ukingire umuco n’ubukonje)
Ugucungera utugingo ngengabuzima	Iyunguruwe biciye mu ruhu rwa 0.2 μm

Gukoresha n'Ibikorwa

Isesengura ry'itandukaniro rya WNR:Iterwa mu cumba c’ugusubiza ca WNR kugira ngo ishireko ikimenyetso c’utugingo ngengabuzima dutukura n’utugingo ngengabuzima twera inyuma y’aho cytoplasme isenyukiye.

WDF 5-Igice WBC Gutandukanya:Ikorana n’imigwi mito mito ya WBC mu muyoboro wa WDF, igatuma habaho amatsinda atandukanye y’umuco w’amaraso y’amaraso, y’amaraso amwe, y’amaraso y’amaraso n’amaraso y’amaraso.

Imbere n'Igitero c'Ibendera:Ivyo bikoresho vyakozwe kugira ngo bihitemwo kwinjira mu bihimba vy’amaraso vyahinduwe mu muhora wa WPC, bikaba vyemeza ko umuntu ashobora kumenya utugingo ngengabuzima tutarakura.

Igitigiri ca ARN y'imitsi y'umubiri:Ifatanya na ARN ya ribosome y’umubiri mu bihimba vy’umubiri bita reticulocytes, bikaba bituma umuntu ashobora kubara inzira z’ugukura kw’ibihimba vy’umubiri bita reticulocytes.

Guharura utugingo ngengabuzima tw'amaso:Itera amabara ibice vya ARN vyo mu mutima vy’amaraso kugira ngo bivemwo igiharuro c’amaso mu bihe vy’ubuvuzi vy’amaraso make-.

Ibipapuro / Ububiko

Umutwe	Ibisobanuro
Gupfunyika bisanzwe	12 mL x 1 icupa / agasanduku, 42 mL x 1 icupa / agasanduku
Icupa ry'ibikoresho	Umuco-amacupa ya HDPE afise fluorine
Ubushuhe bwo kubika	2 degre kugeza kuri degre 30
Ibisabwa mu gutwara	Ukingire ubukonje n'umuco w'izuba

Ivyuma bifitaniye isano n'ivy'amaraso

Umuti w’Ivyuma vy’Ibice 5 vy’Isesengura ry’Amaraso:Isotonic conductive buffer umuti wo gukuraho amaraso no guhagarika urusaku rw’inyuma.

Igikoresho co gupima amaraso mu bice 5:Surfactant-ishimikiye ku muti w’ugusenyuka kw’imitsi y’amaraso.

Umuti wo gusukura wibanze:Igikoresho co gusukura amazi co gucungera umurongo w'amazi no gukuraho ibisigazwa vya proteine.

Igisukura c'itohoza:Igikoresho co gusukura ivyuma vy’ivyuma vy’ivyuma vy’ivyuma vy’ivyuma vy’ivyuma vy’ivyuma vy’ivyuma.

IBIBAZO bibazwa

Ikibazo: Ni gute igikoresho co gukoresha ibara ry’umuco gitandukanye n’ibikoresho vya kera vyo gutera ibara ry’amaraso?

I: Amabara y’ibara ry’agahama asaba kwumishwa n’amaboko ku bipande vy’ibirahuri kugira ngo umuntu asuzume n’amaso akoresheje igikoresho citwa microscope. Ivyo bikoresho vy’ugusesangura irangi vy’amazi ni ivyuma vy’irangi vy’amazi bikoreshwa mu vyumba vy’ugusubiza ibintu mu murongo. Bifatanya na ARN/ADN y’ingingo ngenderwako mu mazi, bikazana ibimenyetso vy’umuco bimenyekana biciye ku buhinga bwa laser flow cytometry.

Ikibazo: Mbega gushikirwa n’umuco biragira ico bikoze ku mikorere y’irangi mu gihe c’ibikorwa vya misi yose?

A: Ego. Ivyo bita fluorophores bikora birasenyuka iyo bimaze igihe kirekire bishikiwe n’umuco canke umuco w’izuba (UV). Ivyo bikoresho bikoreshwa mu gupima bishirwa mu bikoresho vy’umuco-birinzwe n’ibara ry’agahama kandi bitegerezwa kuguma mu gice c’ibikoresho vy’ugupima gipfutse mu gihe c’ibikorwa.

Ikibazo: Ni ubuhe bushuhe bwo kubika busabwa mu gihe co gutwara no kubika?

I: Bika ibikoresho hagati ya degre 2 na degre 30 . Ugubika munsi ya degre 0 bituma haba imvura y’umuyagankuba (fluorophore). Ubushuhe buri hejuru ya degre 35 bwihuta gusenyuka kw’ibirungo bikora.

Ikibazo: Ni gute ukuntu ukuntu -ku -uguhuza bigira ico bikoze ku bisubizo vy'ibara?

I: Uguhinduka kw’irangi bitera uguhinduka kw’ibice vy’irangi, bikaba bituma haba amakosa yo gufunga. Igice cose c’ibikorwa gisuzumwa ubusuku bw’ivyuma bita fluorophore, igerageza ry’ivyuma bita fluorophore, n’uguhuza ivyuma kugira ngo bigumye bifise ikibanza kimwe.

Ikibazo: Ni ubuhe buryo bwo gupfunyika bukoreshwa mu guhuza amazi?

I: Ivyo gutera ibara bitangwa mu macupa ya HDPE afise fluorine afise ibikoresho vy’ugupfuka bigenewe imirongo y’amazi, bikabuza guhinduka umwuka no kwanduza.

Imisoro ishushe: ibara ry’ibikoresho vyo gupima amaraso vy’ibice 5 vya mindray, ibara ry’Ubushinwa ry’ibikoresho vyo gupima amaraso vy’ibice 5 vy’imindray abahinguzi, ababitanga